Skip to contents

Finding cell doublets, with two methods : scds and scDblFinder

Usage

find_doublets(
  sobj,
  assay = "RNA",
  min_clust_size = 100,
  BPPARAM = BiocParallel::SerialParam(),
  my_seed = 1337L,
  verbose = FALSE
)

Arguments

sobj

A Seurat object

assay

CHARACTER : name of the assay corresponding to the initial input data (default to 'RNA')

min_clust_size

INTEGER : minimal cluster size for overclustering steps (see scran/scater help) (default to 100)

BPPARAM

an object of class DoparParam to be passed to BPPARAM parameter of scran::quickCluster and scran::computeSumFactors functions. Could be generate by our create.parallel.instance function

my_seed

INTEGER : random seed (default to 1337L)

verbose

LOGICAL : whether to print progress bars and output messages or not (default to FALSE)

Value

Return the input Seurat object with cell doublets labeled